Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system.
نویسندگان
چکیده
Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.
منابع مشابه
Microcolony cultivation on a soil substrate membrane system selects for previously uncultured soil bacteria.
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 75 10 شماره
صفحات -
تاریخ انتشار 2009